Bacteria resistant to methylmercury utilize two enzymes (MerA and MerB) to degrade methylmercury to the less toxic elemental mercury. The crucial step is the cleavage of the carbon-mercury bond of methylmercury by the organomercurial lyase (MerB). In this study, we determined high resolution crystal structures of MerB in both the free (1.76-A resolution) and mercury-bound (1.64-A resolution) st...

The role of methionine enkephalin (ME) neurons in the development of genetic hypertension in SHR is the subject of this study. Methionine enkephalin-like immunoreactivity (MELI) and ME receptor binding (MERB) levels were assayed quantitatively by microdensitometry of fluorescence micrographs and autoradiographs of 85 cerebral nuclei and areas of both young and adult spontaneously hypertensive r...

We propose that the strong millisecond extragalactic radio burst (mERB) discovered by Lorimer et al. (2007) may be related to a hyperflare from an extragalactic soft gamma-ray repeater. The expected rate of such hyperflares, ∼ 20 50 d Gpc, is in good correspondence with the value estimated by Lorimer et al. The possible mechanism of radio emission can be related to the tearing mode instability ...

In this paper we propose a novel class of methods for high-order accurate integration multirate systems ordinary differential equation initial-value problems. The proposed construct schemes by approximating the action matrix $\varphi$ functions within explicit exponential Rosenbrock (ExpRB) methods, thereby called ExpRB (MERB) methods. They consist solution to sequence modified “fast” problems,...

Methylmercury is an environmental toxicant that biomagnifies and causes severe neurological degeneration in animals. It is produced by bacteria in soils and sediments that have been contaminated with mercury. To explore the potential of plants to extract and detoxify this chemical, we engineered a model plant, Arabidopsis thaliana, to express a modified bacterial gene, merBpe, encoding organome...

El modelamiento in silico ha sido de gran contribución en los procesos proteómicos, desarrollando estructuras las secuencias proteicas ya existentes, que por motivos altos costos y diferentes tecnologías necesarias para el desarrollo estas metodologías, se encuentran deficientes número modelamientos proteínas disponibles. Entre aquellas con carencia estructura proteica encuentra la proteína lia...

We describe theoretical insights into the mechanism of Hg-C bond protonolysis in methyl mercury coordinated by the tris(2-mercapto-1-tert-butylimidazolyl)hydroborato ligand, the structural and functional analogue of the organomercurial lyase MerB. Different cleavage pathways including both frontside and backside attack transition states were systematically studied by the hybrid density function...